The second BIO·FICTION Science Art Film Festival took place from 23 - 25 October, 2014. The international synthetic biology festival was organised by Vienna-based research and science communication company Biofaction with the support of the Museum of Natural History in Vienna, Austria. BIO·FICTION discussed, documented and explored the emerging field of synthetic biology and initiated a public discourse on this current and highly relevant topic. In order to facilitate this creative conversation, the event featured a large variety of content: 32 presentations, panel discussions, 5 do-it-yourself biology demos, 3 art performances, and of course film screenings. The participants came from a wealth of backgrounds, including science and engineering, the social sciences, cultural studies, amateur biology, the military, film making, art and design.